Last data update: 24 January 2024 16:39 CET
Host name: Mycobacterium smegmatis MC2 155 groEL1ΔC (LMBP 13763)
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| Species: | Mycobacterium smegmatis |
| Strain designation: | MC2 155 groEL1ΔC |
| Accession number: | LMBP 13763 |
| Depositor: | This host strain was deposited by Prof. Dr M. Wilmanns1 and K. Beckham1. It was constructed by Dr E. Noens1. 1 European Molecular Biology Laboratory EMBL, Hamburg, Germany |
| Containment level: | This strain has been assigned the containment level Class 2 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | 7H9 (s: 7H10) + OADC |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | - |
| Phenotype: | - |
| Properties: | The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. To improve purification efficiency and yield of polyhistidine-tagged mycobacterial target proteins, the GroEL1ΔC mutant was created by removing the coding sequence for the histidine-rich C-terminus. |
| Further information: | There is a genome sequence of the parental strain Mycobacterium smegmatis MC2 155 available at Genbank (Accession number: NC_008596.1). |
| Original reference: | Noens et al., BMC Biotechnol. 11 (2011), 27 [PMID: 21439037] [DOI: 10.1186/1472-6750-11-27] |
| Restricted use: | - BCCM/MTA adapted by EMBL - Restricted to academic users - Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL |
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Refer in your Materials and Methods: |
Mycobacterium smegmatis MC2 155 groEL1ΔC (LMBP 13763) is available at BCCM/GeneCorner. It was deposited by Prof. Dr M. Wilmanns and K. Beckham and was published in Noens et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.